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Il en résulte un contrôle qualité fenty puma accru et une amélioration du rendement. Ces techniques peuvent être facilement adaptées pour une production à l'échelle commerciale.Les moules en polydiméthylsiloxane (PDMS) ont été préparés selon les procédures décrites précédemment 18 à partir d'un maître de moulage qui a été préparé par gravure ionique réactive profonde (DRIE) d'une tranche de silicium et ensuite silanisé avec (tridécafluoro- 1,1,2,2-tét-rahydrooctyl) trichlorosilane pendant une nuit. La résine PUMA (Dymax 140-M, Torrington, CT) a été distribuée à une épaisseur de 3 mm sur le moule PDMS, puis recouverte d'une feuille de cellophane collée sur un support transparent en polypropylène (épaisseur de 8 mil) pour empêcher l'inhibition par l'oxygène de la croix -réaction de liaison (Fig. 1A).
Une fois sorti du moule, le substrat PUMA a été collé de manière conforme à une autre lamelle de verre revêtue de PUMA (durci) avec une légère pression mécanique. Cette liaison conforme a été convertie en liaison permanente en plaçant la puce PUMA sous la source d'inondation UV pendant 10 minutes supplémentaires (A) Présentation du matériau pendant le moulage puma fenty de la puce PUMA. 1: moule PDMS; 2: résine PUMA; 3: Poteaux PTFE; 4: feuille de polypropylène; 5: cellophane (ou Aclar). (B) Deux méthodes d'interfaçage de la puce PUMA avec des tubes externes: 1: puce PUMA; 2: connecteur coudé; 3: tube en polyuréthane; 4: résine supplémentaire pour colmater la fuite (Dymax 186M); 5: tube PTFE; 6: thermorétractable polyoléfine; puma suede femme 7: bague de retenue.
Le côté droit de la figure 1B illustre l'interfaçage d'un tube en PTFE de 1/16 de pouce (ou de dimensions équivalentes comme un tube PE100 de Becton Dickinson) avec une puce PUMA. Nous avons constaté que les tubes conventionnels en polyéthylène (PE) (par exemple PE100), qui sont couramment utilisés pour s'interfacer avec les dispositifs microfluidiques à base de PDMS, ne fonctionnaient pas bien avec les puces PUMA, car (1) les surfaces en polyéthylène sont résistantes au collage, et (2 ) les tubes hautement élastiques s'effondrent facilement lorsqu'ils sont tirés dans le sens longitudinal, provoquant une délamination de l'adhésif.
La meilleure tubulure parmi celles évaluées était la tubulure en PTFE OD de 1/16 de pouce. Bien puma basket qu'il soit presque impossible de se lier chimiquement aux tubes en PTFE, cela peut être contourné en couvrant la surface externe d'un polyoléfine thermorétractable. Ensuite, le tube en PTFE peut être inséré soit directement dans un trou de 1/16 po de diamètre et fixé avec de la résine supplémentaire, soit dans un trou de 1/8 po avec un tube en PU supplémentaire (1/8 po de diamètre extérieur) comme ancrage de cisaillement , et enfin fixé avec de la résine supplémentaire.
Nous avons également exploité les différences de dilatation thermique (par exemple, soit une congélation rapide à -80 ° C ou de la chaleur): le traitement thermique a provoqué une déformation du PDMS dans une direction opposée à la résine durcie, mais la résine durcie s'est également globalement conformée au PDMS déformé. Le résultat était une résine PUMA déformée, rendant impossible le scellement conforme suivant sur un substrat plan. Méthodes de liaison des puces PUMA pour former des canaux fermés. Une puce PUMA, composée d'un morceau d'une réplique PUMA et d'une lamelle recouverte de PUMA, peut être liée soit à l'aide de plasma d'oxygène, suivie d'une cuisson prolongée (à gauche), soit liée de manière conforme à l'aide d'un scellant sous vide, suivie d'une liaison UV / thermique , Collage IR ou soudage par ultrasons (à droite).
En raison de la rigidité du substrat, l'étanchéité conforme de PUMA n'est pas aussi simple que celle de PDMS; des précautions doivent être prises pour éviter de piéger les bulles d'air. De plus, une pression excessive puma rihanna pourrait endommager les microstructures. Notre méthode préférée était de placer la puce dans un sac en plastique, d'utiliser un scellant sous vide qui est vendu dans le commerce comme un appareil de cuisine pour tirer le vide sur le sac et de s'appuyer sur le sac qui s'effondre pour appliquer une force de compression uniforme sur la puce pour former le joint conforme. Les sacs en plastique destinés à être scellés sous vide ont souvent des arêtes pour réduire l'effondrement prématuré du sac avant que l'air ne soit complètement évacué; ces crêtes peuvent laisser des empreintes sur le substrat PUMA.
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